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question:You are an AI assistant. Provide a detailed answer so user don’t need to search outside to understand the answer. Detailed Instructions: Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage. Problem:We demonstrated previously that striatal adenosine modulates ethanol-induced motor incoordination (EIMI) via adenosine A1 receptors coupled to pertussis toxin (PT)-sensitive G protein and adenylyl cyclase-cyclic adenosine monophosphate (cAMP). Additionally, intrastriatal (IST) PT antagonizes EIMI and its potentiation by the adenosine A1 agonist N-cyclohexyladenosine; it also inhibits cAMP concentration.', 'Guide cannulas were stereotaxically implanted for IST pretreatment with PT followed 5 days later by IST of N-cyclohexyladenosine and intraperitoneal ethanol. The adenosine diphosphate (ADP) ribosylation reaction involved PT-catalyzed [P]nicotinamide adenine dinucleotide (NAD) labeling of rat striatal membranes. Antagonism of EIMI (Rotorod method) after IST microinfusion of PT was investigated to determine whether it was due to a decrease in the functional activity of G proteins due to ADP ribosylation of the Gialpha subunit caused it.', 'Striatal membranes from IST PT (0.5 microg)-treated animals exhibited significantly attenuated (up to 90%) in vitro ADP ribosylation with [P]NAD. Striatal membranes from animals injected with ethanol (1.5 g/kg intraperitoneally) exhibited statistically significant increase (11%) in in vitro ADP ribosylation. Similarly, ethanol (50 mM) added to striatal membranes from untreated animals produced significant stimulation of in vitro ADP ribosylation. The decrease in the functional activity of G proteins due to ADP ribosylation of the Gialpha subunit after IST PT was functionally correlated with marked attenuation in EIMI, as observed previously. This finding suggests a blockade of functional activity of PT-sensitive striatal Gi/Go proteins (i.e., fewer available sites for labeled NAD incorporation). The in vivo ethanol results indicate that it must have caused an increase in the ribosylation capacity of Gialpha in vivo (i.e., increased Gi activity). Increased ADP ribosylation by in vitro ethanol increases Gi/Go activity, consistent with EIMI, as previously reported. Solution:
answer:Question: What was the observed effect of intrastriatal pertussis toxin treatment on adenosine diphosphate (ADP) ribosylation in rat striatal membranes and its correlation to ethanol-induced motor incoordination (EIMI)? Answer: In the study, striatal membranes from intrastriatally pertussis toxin (IST PT) treated animals exhibited a significantly attenuated, up to 90%, in vitro ADP ribosylation. This decrease in functional activity of G proteins due to ADP ribosylation of the Gialpha subunit after IST PT was functionally correlated to a marked attenuation in ethanol-induced motor incoordination. This suggested a blockade in functional activity of PT-sensitive striatal Gi/Go proteins due to the fewer available sites for labeled NAD incorporation. Additionally, in vivo ethanol results indicated that it caused an increase in the ribosylation capacity of Gialpha in vivo (i.e., an increased Gi activity). An increase in ADP ribosylation by in vitro ethanol resulted in an increase in Gi/Go activity, consistent with EIMI, as previously reported.
question:You are a helpful assistant, who always provide explanation. Think like you are answering to a five year old. În martie, o rezoluţie a Parlamentului European v-a solicitat să reduceţi negocierile ACTA privind contrafacerea. Could you please translate this to English?
answer:In March, a resolution of the European Parliament asked you to reduce the ACTA negotiations on counterfeiting. In simpler words, a big group in Europe wanted to have less talks on ACTA, which is about stopping fake things and copying stuff.
question:You are an AI assistant that follows instruction extremely well. Help as much as you can. What is the answer for the question: What is the name of the attack that Terry Nichols helped plan, and for which he is serving life in prison without parole? from the following article ? Social tensions were exacerbated by the revival of the Ku Klux Klan after 1915. The Tulsa Race Riot broke out in 1921, with whites attacking blacks. In one of the costliest episodes of racial violence in American history, sixteen hours of rioting resulted in 35 city blocks destroyed, 1.8 million in property damage, and a death toll estimated to be as high as 300 people. By the late 1920s, the Ku Klux Klan had declined to negligible influence within the state.During the 1930s, parts of the state began suffering the consequences of poor farming practice. This period was known as the Dust Bowl, throughout which areas of Kansas, Texas, New Mexico and northwestern Oklahoma were hampered by long periods of little rainfall, strong winds, and abnormally high temperatures, sending thousands of farmers into poverty and forcing them to relocate to more fertile areas of the western United States. Over a twenty-year period ending in 1950, the state saw its only historical decline in population, dropping 6.9 percent as impoverished families migrated out of the state after the Dust Bowl. Soil and water conservation projects markedly changed practices in the state and led to the construction of massive flood control systems and dams; they built hundreds of reservoirs and man-made lakes to supply water for domestic needs and agricultural irrigation. By the 1960s, Oklahoma had created more than 200 lakes, the most in the nation.In 1995, Oklahoma City was the site of one of the most destructive acts of domestic terrorism in American history. The Oklahoma City bombing of April 19, 1995, in which Timothy McVeigh detonated a large, crude explosive device outside the Alfred P. Murrah Federal Building, killed 168 people, including 19 children. For his crime, McVeigh was executed by the federal government on June 11, 2001. His accomplice, Terry Nichols, is serving life in prison without parole for helping plan the attack and prepare the explosive.On May 31, 2016, several cities experienced record setting flooding. Answer:
answer:The Oklahoma City bombing
question:You are an AI assistant. User will you give you a task. Your goal is to complete the task as faithfully as you can. While performing the task think step-by-step and justify your steps. OPTIONS: i. Someone who cares about their mother. ii. angry next. iii. responsible afterwards. Given those answer options, answer the question: I heard that Sasha saved her mother's belongings for when she returned from the hospital. And I was wondering How would Sasha feel afterwards? A:
answer:Based on the given options, I would select: i. Someone who cares about their mother. Step by step justification: 1. The question asks about Sasha's feelings after saving her mother's belongings for when she returns from the hospital. 2. The information given implies that Sasha has taken the time and effort to preserve her mother's belongings, indicating care and concern for her mother. 3. Considering the given response options, the one that best matches Sasha's actions is "Someone who cares about their mother." 4. The other two options, "angry next" and "responsible afterwards," do not seem relevant to the described act of caring and do not accurately represent Sasha's feelings in this context. Therefore, Sasha would feel like someone who cares about their mother after saving her belongings for when she returns from the hospital.